VirB alleviates H-NS repression of the icsP promoter in Shigella flexneri from sites more than one kilobase upstream of the transcription start site

J Bacteriol. 2009 Jun;191(12):4047-50. doi: 10.1128/JB.00313-09. Epub 2009 Apr 10.

Abstract

The icsP promoter of Shigella spp. is repressed by H-NS and derepressed by VirB. Here, we show that an inverted repeat located between positions -1144 and -1130 relative to the icsP transcription start site is necessary for VirB-dependent derepression. The atypical location of this cis-acting site is discussed.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Down-Regulation*
  • Gene Expression Regulation, Bacterial
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Shigella flexneri / enzymology
  • Shigella flexneri / genetics*
  • Shigella flexneri / metabolism
  • Transcription Initiation Site*
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • H-NS protein, bacteria
  • Virulence Factors
  • SopA protein, Bacteria