EQCM biosensors based on DNA aptamers and antibodies for rapid detection of prions

Protein Pept Lett. 2009;16(4):363-7. doi: 10.2174/092986609787848090.

Abstract

Novel affinity biosensors for detecting cellular prions, PrP(C), based on DNA aptamers and antibodies immobilized onto the carbon nanotubes have been designed and compared in accordance with their binding ability and analytical performance. The biosensors allowed us to detect PrP(C) with the limits of detection of 20 to 50 pM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / analysis*
  • Aptamers, Nucleotide*
  • Biosensing Techniques / methods*
  • Immobilized Proteins
  • PrPC Proteins / analysis*
  • PrPC Proteins / immunology
  • Prions / analysis*
  • Prions / immunology

Substances

  • Antibodies
  • Aptamers, Nucleotide
  • Immobilized Proteins
  • PrPC Proteins
  • Prions