Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI

Angew Chem Int Ed Engl. 2009;48(20):3641-3. doi: 10.1002/anie.200806328.

Abstract

Dynamic duo: Magnetic resonance imaging (MRI) can visualize deep regions of living bodies, whereas fluorescence measurement offers excellent sensitivity. These methods thus offer signal enhancement potential for detecting enzyme activities. A dual-mode off/on probe to detect caspase-3 activity by fluorescence and (19)F MRI is presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caspase 3 / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorine / chemistry*
  • Luminescent Measurements
  • Nuclear Magnetic Resonance, Biomolecular*
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry*
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*

Substances

  • Fluorescent Dyes
  • Oligopeptides
  • Organometallic Compounds
  • gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid -7-amino-4-trifluoromethylcoumarin
  • gadolinum-DOTA-aspartyl-glutamyl-valyl-aspartic acid 4-trifluoromethoxybenzylamide
  • Fluorine
  • Caspase 3