Elaboration of optical immunosensors based on the surface plasmon resonance for detecting specific antibodies and antigens of Epstein-Barr virus and human adenovirus

N V Nesterova; L M Nosach; S D Zagorodnya; O Y Povnitsa; P M Boltovets; G V Baranova; A V Golovan

Elaboration of optical immunosensors based on the surface plasmon resonance for detecting specific antibodies and antigens of Epstein-Barr virus and human adenovirus

Mikrobiol Z. 2008 Nov-Dec;70(6):67-73.

Authors

N V Nesterova¹, L M Nosach, S D Zagorodnya, O Y Povnitsa, P M Boltovets, G V Baranova, A V Golovan

Affiliation

¹ Zabolotny Institute of Microbiology and Virology, National Academy Sciences of Ukraine 154 Acad. Zabolotny St., Kyiv, MSP, G03680, Ukraine.

PMID: 19351051

Abstract

The study of antigen-antibody interaction on the model of Epstein-Barr virus (EBV) and second type adenovirus (Ad2) based on the surface plasmon resonance (SPR) was carried out. Kinetic and concentration dependences between virus antigens and specific antisera to them at different pH were determined. Experimental samples of biosensors for the detection by SPR method of virus (EBV and Ad2) antigens using monospecific antibodies, immobilized on the surface of gold, and also for detection of specific antibodies in the blood sera of patients with EBV or adenovirus infection were elaborated

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviruses, Human / immunology*
Adenoviruses, Human / isolation & purification
Antibodies, Viral / immunology*
Antigen-Antibody Complex / analysis*
Antigen-Antibody Complex / immunology
Antigens, Viral / immunology*
Biosensing Techniques / methods*
Cell Line, Tumor
Herpesvirus 4, Human / immunology*
Herpesvirus 4, Human / isolation & purification
Humans
Immunoenzyme Techniques
Microscopy, Electron
Sensitivity and Specificity
Spectrophotometry
Surface Plasmon Resonance

Substances

Antibodies, Viral
Antigen-Antibody Complex
Antigens, Viral