Abstract
USPIO-enhanced MRI allows non-invasive visualization of mononuclear cell infiltration into CNS lesions in MS and EAE. Herein, we show a distinct spatiotemporal pattern of CNS lesions that reveals the involvement of spino-olivocerebellar pathways in MOG-induced EAE rats using USPIO-enhanced MRI. Specifically, lesions of the inferior olives were observed primarily in the acute phase whereas lesions of cerebellum or spinal cord/brainstem were observed during the relapse phase. Further, behavioral deficits observed from these animals are consistent with the functional role of spino-olivocerebellar pathways in coordination and movement. Collectively, our results provide new insights into the pathophysiology of this animal model of MS.
MeSH terms
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Animals
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Brain / pathology*
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Cerebellum / pathology
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Dextrans
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Encephalomyelitis, Autoimmune, Experimental / pathology*
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Female
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Ferrosoferric Oxide
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Immunohistochemistry
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Iron
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Magnetic Resonance Imaging* / methods
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Magnetite Nanoparticles
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Motor Activity / physiology
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Myelin Proteins
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Myelin-Associated Glycoprotein / immunology
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Myelin-Associated Glycoprotein / toxicity
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Myelin-Oligodendrocyte Glycoprotein
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Neural Pathways / pathology*
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Olivary Nucleus / pathology
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Oxides
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Radiopharmaceuticals
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Rats
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Spinal Cord / pathology*
Substances
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Dextrans
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Magnetite Nanoparticles
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Mog protein, rat
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Myelin Proteins
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Myelin-Associated Glycoprotein
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Myelin-Oligodendrocyte Glycoprotein
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Oxides
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Radiopharmaceuticals
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ferumoxtran-10
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Iron
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Ferrosoferric Oxide