Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

Malgorzata Kaczmarek; Stefan Lis

doi:10.1016/j.aca.2009.02.047

Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

Anal Chim Acta. 2009 Apr 20;639(1-2):96-100. doi: 10.1016/j.aca.2009.02.047. Epub 2009 Mar 9.

Authors

Malgorzata Kaczmarek¹, Stefan Lis

Affiliation

¹ Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznań, Poland.

PMID: 19345765
DOI: 10.1016/j.aca.2009.02.047

Abstract

A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4x10(-7) to 2x10(-5) mol L(-1) of Chlor-TC; 2x10(-7) to 2x10(-5) mol L(-1) of Oxy-TC and 1x10(-7) to 3x10(-5) mol L(-1) of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.

MeSH terms

Europium / chemistry*
Honey / analysis
Hydrogen Peroxide / chemistry*
Iron / chemistry*
Kinetics
Luminescence
Luminescent Measurements / methods*
Pharmaceutical Preparations / chemistry
Tetracyclines / analysis*
Tetracyclines / chemistry
Water / chemistry

Substances

Fenton's reagent
Pharmaceutical Preparations
Tetracyclines
Water
Europium
Hydrogen Peroxide
Iron