Characterization of a highly pathogenic avian influenza H5N1 virus sublineage in poultry seized at ports of entry into Vietnam

Tung Nguyen; C Todd Davis; William Stembridge; Bo Shu; Amanda Balish; Kenjiro Inui; Hoa T Do; Huong T Ngo; Xiu-Feng Wan; Margaret McCarron; Stephen E Lindstrom; Nancy J Cox; Cam V Nguyen; Alexander I Klimov; Ruben O Donis

doi:10.1016/j.virol.2009.03.006

Characterization of a highly pathogenic avian influenza H5N1 virus sublineage in poultry seized at ports of entry into Vietnam

Virology. 2009 May 10;387(2):250-6. doi: 10.1016/j.virol.2009.03.006. Epub 2009 Apr 2.

Authors

Tung Nguyen¹, C Todd Davis, William Stembridge, Bo Shu, Amanda Balish, Kenjiro Inui, Hoa T Do, Huong T Ngo, Xiu-Feng Wan, Margaret McCarron, Stephen E Lindstrom, Nancy J Cox, Cam V Nguyen, Alexander I Klimov, Ruben O Donis

Affiliation

¹ National Centre for Veterinary Diagnostics, Department of Animal Health, Hanoi, Vietnam.

PMID: 19342072
DOI: 10.1016/j.virol.2009.03.006

Abstract

Highly pathogenic avian influenza H5N1 virus was detected in poultry seized at two ports of entry located in Lang Son Province, Vietnam. Sequence analysis of the hemagglutinin (HA) genes from five H5N1 virus isolates and ten PCR amplicons from chicken cloacal samples revealed their close phylogenetic relationship to clade 7 H5N1 HA genes. However, these HA genes exhibited extensive genetic divergence at both the nucleotide and amino acid levels in comparison to previously described clade 7 viruses; e.g., A/chicken/Shanxi/2/2006. In addition, hemagglutination inhibition tests revealed antigenic differences between these and previously isolated H5N1 viruses from Vietnam. These results indicate that viruses with clade 7 HA are evolving rapidly in poultry in Southeast Asia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigen-Antibody Reactions
Antigens, Viral / genetics
Antigens, Viral / immunology
Chickens / virology*
Cloaca / virology
Genes, Viral
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / genetics
Influenza A Virus, H5N1 Subtype / classification*
Influenza A Virus, H5N1 Subtype / isolation & purification
Influenza A Virus, H5N1 Subtype / pathogenicity*
Influenza in Birds / epidemiology
Influenza in Birds / virology*
Molecular Sequence Data
Phylogeny
Polymorphism, Single Nucleotide
Poultry Diseases / epidemiology
Poultry Diseases / virology*
Vietnam / epidemiology

Substances

Antigens, Viral
Hemagglutinin Glycoproteins, Influenza Virus
hemagglutinin, avian influenza A virus

Associated data

GENBANK/FJ842476
GENBANK/FJ842480
GENBANK/FJ842481
GENBANK/FJ842482
GENBANK/FJ842483