Synthesis and characterization of circular structures of i-motif tagged with fluoresceins

Bioconjug Chem. 2009 Apr;20(4):644-7. doi: 10.1021/bc8004599.

Abstract

Synthesis of a fluorescein-tagged circular structure of i-motif was accomplished during our investigations. The backbone circularity of this molecular probe and the presence of the fluorescent moiety within its structure were also confirmed through exonuclease hydrolysis and fluorescence spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing
  • Base Sequence
  • Electrophoretic Mobility Shift Assay
  • Fluorescein / chemistry*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Microinjections
  • Molecular Probes / chemical synthesis*
  • Molecular Probes / chemistry*
  • Molecular Probes / genetics
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics
  • Oligonucleotides / metabolism
  • Proteins / metabolism
  • Spectrometry, Fluorescence

Substances

  • Molecular Probes
  • Oligonucleotides
  • Proteins
  • Fluorescein