Direct detection of plant viruses in potato tubers using real-time PCR

Neil Boonham; Lynn Laurenson; Rebecca Weekes; Rick Mumford

doi:10.1007/978-1-59745-062-1_19

Direct detection of plant viruses in potato tubers using real-time PCR

Methods Mol Biol. 2009:508:249-58. doi: 10.1007/978-1-59745-062-1_19.

Authors

Neil Boonham¹, Lynn Laurenson, Rebecca Weekes, Rick Mumford

Affiliation

¹ Central Science Laboratories, Sand Hutton, York, UK.

PMID: 19301760
DOI: 10.1007/978-1-59745-062-1_19

Abstract

Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Clinical Laboratory Techniques
Enzyme-Linked Immunosorbent Assay / methods
Nucleic Acid Amplification Techniques
Plant Viruses / isolation & purification*
Polymerase Chain Reaction / methods*
RNA, Viral / analysis
RNA, Viral / genetics
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Solanum tuberosum / virology*

Substances

RNA, Viral