Abstract
Immunocapture followed by the detection of viruses using polymerase chain reaction is a versatile, sensitive and robust diagnostic technique. The application of this hybrid method of virus detection in plants is particularly useful in species or tissues containing inhibitory substances. In addition, antibody-mediated virus purification is usually simpler than other methods of isolation.
MeSH terms
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Clinical Laboratory Techniques
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DNA Probes
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DNA, Viral / analysis
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Immunoprecipitation / methods*
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Molecular Sequence Data
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Plant Diseases / genetics
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Plant Diseases / virology*
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Plant Viruses / genetics
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Plant Viruses / immunology
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Plant Viruses / isolation & purification*
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Plants / virology
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Polymerase Chain Reaction / methods*
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RNA Viruses / genetics
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RNA Viruses / isolation & purification
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RNA, Viral / analysis
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
Substances
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DNA Probes
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DNA, Viral
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RNA, Viral