Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer

Biotechniques. 2009 Jan;46(1):58-60. doi: 10.2144/000113045.

Abstract

Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Fluorescence Resonance Energy Transfer / methods*
  • GTP-Binding Proteins / genetics*
  • Genotype*
  • Ghana
  • Humans
  • Microsatellite Repeats*
  • Molecular Sequence Data
  • Promoter Regions, Genetic*

Substances

  • GTP-Binding Proteins
  • IRGM protein, human