We proposed the analyses of polyphenolic coumpounds from the Melissa officinalis L. (Lamiaceae) leaves obtained from Medicinal Plant Collection of USAMV Cluj-Napoca.
Material and method: The study was performed by using spectrophotometric (I) and HPLC methods (II).
Results: The vegetal product contains 0.64% flavonoids expressed in rutoside and 8,962% phenyl-propane derivatives expressed in caffeic acid (I). HPLC analyses (II) were made after extraction of studied compounds from leaves with ethyl-ether, ethyl acetate and 1-buthanol. These extracts were analyzed before and after the hydrolysis of compounds. There were identified 6 polyphenolic compounds: caftaric acid, caffeic acid, p-cumaric acid, ferulic acid, luteolin and apigenin.
Conclusion: The extracted amount of these compounds in chosen solvents depending of their polarity.