Tryptophan 13C nuclear-spin polarization generated by intraprotein electron transfer in a LOV2 domain of the blue-light receptor phototropin

Biochem Soc Trans. 2009 Apr;37(Pt 2):382-6. doi: 10.1042/BST0370382.

Abstract

(13)C-NMR experiments were performed on photo-excited fully and partially (13)C-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from (13)C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN (13)C isotopologues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Carbon Isotopes
  • Cryptochromes
  • Electron Transport
  • Flavin Mononucleotide / chemistry
  • Flavin Mononucleotide / radiation effects
  • Flavoproteins / chemistry*
  • Flavoproteins / radiation effects
  • Light
  • Magnetic Resonance Spectroscopy
  • Protein Structure, Tertiary
  • Tryptophan / chemistry*

Substances

  • Carbon Isotopes
  • Cryptochromes
  • Flavoproteins
  • Flavin Mononucleotide
  • Tryptophan