Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods

J Plutzer; P Karanis

doi:10.1007/s00436-009-1391-3

Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods

Parasitol Res. 2009 Jun;104(6):1527-33. doi: 10.1007/s00436-009-1391-3. Epub 2009 Mar 14.

Authors

J Plutzer¹, P Karanis

Affiliation

¹ National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. plujud@yahoo.com

PMID: 19288133
DOI: 10.1007/s00436-009-1391-3

Abstract

The detection of two human-pathogenic Giardia duodenalis assemblages A and B in faecal and water samples by loop-mediated isothermal amplification (LAMP) has been evaluated. The LAMP reaction is reproducible, rapid and specific for the detection G. duodenalis and has lower costs compared to the other molecular assays. This is the first application of LAMP for Giardia detection.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Environmental Microbiology*
Feces / parasitology
Giardia / genetics
Giardia / isolation & purification*
Humans
Nucleic Acid Amplification Techniques / economics
Nucleic Acid Amplification Techniques / methods*
Sensitivity and Specificity
Time Factors