Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308

Bioprocess Biosyst Eng. 2009 Oct;32(6):837-43. doi: 10.1007/s00449-009-0310-4. Epub 2009 Mar 12.

Abstract

Docosahexaenoic acid (DHA) production in Schizochytrium sp. HX-308 was evaluated by detecting enzymatic activities of ATP:citrate lyase (EC 4.1.3.8), malic enzyme (EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) at different fermentation stages. According to the analysis, a regulation strategy was proposed which reinforced acetyl-CoA and NADPH supply at a specific fermentation stage. DHA content of total fatty acids was increased from 35 to 60% by the addition of 4 g/L malic acid at the rapid lipid accumulation stage. Total lipid content also showed an apparent increase of 35% and reached 19 g/L when 40 mL ethanol/L was added at the late lipid accumulation stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Citrate (pro-S)-Lyase / metabolism
  • Acetyl Coenzyme A / metabolism
  • Bioreactors / microbiology
  • Biotechnology
  • Culture Media
  • Docosahexaenoic Acids / metabolism*
  • Eukaryota / growth & development
  • Eukaryota / metabolism*
  • Eukaryotic Cells / metabolism*
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism
  • Fermentation
  • Gas Chromatography-Mass Spectrometry
  • Kinetics
  • Malate Dehydrogenase / metabolism
  • NADP / metabolism

Substances

  • Culture Media
  • Fatty Acids
  • Docosahexaenoic Acids
  • NADP
  • Acetyl Coenzyme A
  • Malate Dehydrogenase
  • malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
  • ATP Citrate (pro-S)-Lyase