Abstract
CX(3)CR1 expression is associated with the commitment of CSF-1R(+) myeloid precursors to the macrophage/dendritic cell (DC) lineage. However, the relationship of the CSF-1R(+) CX(3)CR1(+) macrophage/DC precursor (MDP) with other DC precursors and the role of CX(3)CR1 in macrophage and DC development remain unclear. We show that MDPs give rise to conventional DCs (cDCs), plasmacytoid DCs (PDCs), and monocytes, including Gr1(+) inflammatory monocytes that differentiate into TipDCs during infection. CX(3)CR1 deficiency selectively impairs the recruitment of blood Gr1(+) monocytes in the spleen after transfer and during acute Listeria monocytogenes infection but does not affect the development of monocytes, cDCs, and PDCs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology
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CX3C Chemokine Receptor 1
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Cell Differentiation
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Cell Movement
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Cell Proliferation
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Cell Survival
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Dendritic Cells / cytology
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Dendritic Cells / enzymology*
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Dendritic Cells / immunology
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Inflammation / enzymology*
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Inflammation / immunology
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Listeria monocytogenes
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Listeriosis / immunology
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Macrophages / cytology
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Macrophages / enzymology*
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Macrophages / immunology
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Mice
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Monocytes / cytology
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Monocytes / immunology
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Nitric Oxide Synthase Type II / metabolism
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Phenotype
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Reactive Oxygen Species / metabolism
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Receptor, Macrophage Colony-Stimulating Factor / metabolism*
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Receptors, Chemokine / deficiency
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Receptors, Chemokine / metabolism*
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Spleen / cytology
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Spleen / immunology
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Spleen / microbiology
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Stem Cells / cytology
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Stem Cells / enzymology*
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Stem Cells / immunology
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Tumor Necrosis Factor-alpha / biosynthesis
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fms-Like Tyrosine Kinase 3 / metabolism*
Substances
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CX3C Chemokine Receptor 1
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Cx3cr1 protein, mouse
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Reactive Oxygen Species
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Receptors, Chemokine
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Tumor Necrosis Factor-alpha
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Nitric Oxide Synthase Type II
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Receptor, Macrophage Colony-Stimulating Factor
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fms-Like Tyrosine Kinase 3