Synthesis, characterization, and bioconjugation of fluorescent gold nanoclusters toward biological labeling applications

Cheng-An J Lin; Ting-Ya Yang; Chih-Hsien Lee; Sherry H Huang; Ralph A Sperling; Marco Zanella; Jimmy K Li; Ji-Lin Shen; Hsueh-Hsiao Wang; Hung-I Yeh; Wolfgang J Parak; Walter H Chang

doi:10.1021/nn800632j

Synthesis, characterization, and bioconjugation of fluorescent gold nanoclusters toward biological labeling applications

ACS Nano. 2009 Feb 24;3(2):395-401. doi: 10.1021/nn800632j.

Authors

Cheng-An J Lin¹, Ting-Ya Yang, Chih-Hsien Lee, Sherry H Huang, Ralph A Sperling, Marco Zanella, Jimmy K Li, Ji-Lin Shen, Hsueh-Hsiao Wang, Hung-I Yeh, Wolfgang J Parak, Walter H Chang

Affiliation

¹ Department of Biomedical Engineering, Chung Yuan Christian University, Chung-Li 32023, Taiwan, ROC.

PMID: 19236077
DOI: 10.1021/nn800632j

Abstract

Synthesis of ultrasmall water-soluble fluorescent gold nanoclusters is reported. The clusters have a decent quantum yield, high colloidal stability, and can be readily conjugated with biological molecules. Specific staining of cells and nonspecific uptake by living cells is demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cell Line, Tumor
Cell Survival
Chromatography, Gel
Endothelial Cells / cytology
Endothelial Cells / metabolism
Fluorescent Dyes / chemical synthesis*
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism
Gold / chemistry*
Humans
Metal Nanoparticles / chemistry*
Polyethylene Glycols / chemistry
Serum Albumin, Bovine / chemistry
Serum Albumin, Bovine / metabolism
Spectrophotometry, Ultraviolet
Staining and Labeling / methods*
Substrate Specificity

Substances

Fluorescent Dyes
Serum Albumin, Bovine
Polyethylene Glycols
Gold