Objective: To study the conditions of extraction of active fraction in Rhizoma et Radix Ligustici by supercritical CO2 extraction (SFE).
Methods: Using the content of ferulic acid as the index, conditions for the extraction including extracting pressure, extracting temperature, temperature of resolution and extracting time were optimized by uniform design.
Results: The best SFE conclusions were as follows: cosolvent was 50% ethanol (ml/g), extracting pressure was 40 MPa, extracting temperature was 50 degrees C, pressure of resolution was 6 MPa, temperature of resolution was 50 degrees C, extracting time was 3.5 hours.
Conclusions: The optimum extraction has high extraction ratio of active fraction in Rhizoma et Radix Ligustici, so it is reasonable and practicable.