Activity-guided isolation of antioxidant principles from Limoniastrum feei (Girard) Batt

Abstract

Bioguided fractionation of a leaves extract from Limoniastrum feei (Girard) Batt. (Plumbaginaceae) led to the isolation of seven polyphenolic constituents: gallic acid (1), myrciaphenone A (2), myricetin-3-O-beta-galactopyranoside (3-1), epigallocatechin gallate (3-2), myricetin 3-O-alpha-rhamnopyranoside (4), quercetin (5) and myricetin (6). Gallic acid was the most antioxidant compound in DPPH [(0.94 +/- 0.68) microg/mL] and FRAP [(0.83 +/- 0.15) microM Fe2+/mL] tests, whereas myricetin was a more specific superoxide radical scavenger since it was the most active product in the superoxide nitroblue tetrazolium hypoxanthine/xanthine oxidase test [(1.86 +/- 0.12) microg/mL].