Relaxation-optimised Hartmann-Hahn transfer using a specifically Tailored MOCCA-XY16 mixing sequence for carbonyl-carbonyl correlation spectroscopy in 13C direct detection NMR experiments

Isabella C Felli; Roberta Pierattelli; Steffen J Glaser; Burkhard Luy

doi:10.1007/s10858-009-9302-6

Relaxation-optimised Hartmann-Hahn transfer using a specifically Tailored MOCCA-XY16 mixing sequence for carbonyl-carbonyl correlation spectroscopy in 13C direct detection NMR experiments

J Biomol NMR. 2009 Mar;43(3):187-96. doi: 10.1007/s10858-009-9302-6. Epub 2009 Feb 18.

Authors

Isabella C Felli¹, Roberta Pierattelli, Steffen J Glaser, Burkhard Luy

Affiliation

¹ Department of Chemistry and Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Florence, Italy.

PMID: 19224374
DOI: 10.1007/s10858-009-9302-6

Abstract

Isotropic mixing sequences are one of the key methods to achieve efficient coherence transfer. Among them, the MOCCA-XY16, which keeps the magnetization longitudinal for a significant amount of time, is characterised by favourable relaxation properties. We show here that its adapted version is particularly suited for carbonyl-carbonyl correlations in (13)C direct detection NMR experiments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence*
Carbon Isotopes / chemistry*
Models, Theoretical
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular / methods*
Proteins / chemistry*
Signal Processing, Computer-Assisted*

Substances

Carbon Isotopes
Nitrogen Isotopes
Proteins