Simple and rapid colorimetric sensing of enzymatic cleavage and oxidative damage of single-stranded DNA with unmodified gold nanoparticles as indicator

Qinpeng Shen; Zhou Nie; Manli Guo; Chuan-Jian Zhong; Bin Lin; Wang Li; Shouzhuo Yao

doi:10.1039/b818081d

Simple and rapid colorimetric sensing of enzymatic cleavage and oxidative damage of single-stranded DNA with unmodified gold nanoparticles as indicator

Chem Commun (Camb). 2009 Feb 28:(8):929-31. doi: 10.1039/b818081d. Epub 2009 Jan 14.

Authors

Qinpeng Shen¹, Zhou Nie, Manli Guo, Chuan-Jian Zhong, Bin Lin, Wang Li, Shouzhuo Yao

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

PMID: 19214319
DOI: 10.1039/b818081d

Abstract

The sequence-length-dependent adsorption of single-stranded DNA (ssDNA) on unmodified gold nanoparticles is exploited as a new mechanism for colorimetric nuclease assay and measurement of oxidative DNA damage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Colorimetry / methods*
DNA Damage*
DNA, Single-Stranded / chemistry*
Gold / chemistry*
Indicators and Reagents
Metal Nanoparticles / chemistry*
Oxidation-Reduction
Time Factors

Substances

DNA, Single-Stranded
Indicators and Reagents
Gold