2-Aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

Minjie Li; Yusuke Sato; Seiichi Nishizawa; Takehiro Seino; Kodai Nakamura; Norio Teramae

doi:10.1021/ja8095625

2-Aminopurine-modified abasic-site-containing duplex DNA for highly selective detection of theophylline

J Am Chem Soc. 2009 Feb 25;131(7):2448-9. doi: 10.1021/ja8095625.

Authors

Minjie Li¹, Yusuke Sato, Seiichi Nishizawa, Takehiro Seino, Kodai Nakamura, Norio Teramae

Affiliation

¹ Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.

PMID: 19191489
DOI: 10.1021/ja8095625

Abstract

2-Aminopurine-modified abasic-site-containing duplex [DNA 5'-TCTGC GTCCT PXT TAACG CACAC-3'/3'-AGACG CAGGA TCA ATTGC GTGTG-5'; P = 2-aminopurine, X = abasic site (Spacer-C3), C = receptor base] is capable of selectively binding to the bronchodilator theophylline with a dissociation constant of 10 microM (5 degrees C, pH 7.0, I = 0.11 M) and is applicable to monitoring serum theophylline concentrations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2-Aminopurine / chemistry*
Aptamers, Nucleotide / chemical synthesis
Aptamers, Nucleotide / chemistry*
DNA / chemistry*
DNA Probes / chemistry
Fluorescent Dyes / chemistry
Kinetics
Models, Molecular
Nucleic Acid Conformation
Spectrometry, Fluorescence / methods
Theophylline / analysis*
Theophylline / chemistry

Substances

Aptamers, Nucleotide
DNA Probes
Fluorescent Dyes
2-Aminopurine
DNA
Theophylline