Stepwise synthesis of RNA conjugates carrying peptide sequences for RNA interference studies

Mol Divers. 2009 Aug;13(3):287-93. doi: 10.1007/s11030-009-9110-7. Epub 2009 Feb 3.

Abstract

Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3'-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at the 3'-end of the passenger strand has similar inhibitory properties as those of unmodified or cholesterol-modified RNA duplexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Chromatography, High Pressure Liquid
  • Enzyme-Linked Immunosorbent Assay
  • HeLa Cells
  • Humans
  • Nuclear Localization Signals / chemical synthesis*
  • Nucleoplasmins / genetics
  • Oligoribonucleotides / chemical synthesis*
  • Peptides / chemical synthesis*
  • RNA Interference*
  • RNA, Small Interfering / chemical synthesis*
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Nuclear Localization Signals
  • Nucleoplasmins
  • Oligoribonucleotides
  • Peptides
  • RNA, Small Interfering
  • Tumor Necrosis Factor-alpha