Abstract
Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3'-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at the 3'-end of the passenger strand has similar inhibitory properties as those of unmodified or cholesterol-modified RNA duplexes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Chromatography, High Pressure Liquid
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Enzyme-Linked Immunosorbent Assay
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HeLa Cells
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Humans
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Nuclear Localization Signals / chemical synthesis*
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Nucleoplasmins / genetics
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Oligoribonucleotides / chemical synthesis*
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Peptides / chemical synthesis*
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RNA Interference*
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RNA, Small Interfering / chemical synthesis*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Nuclear Localization Signals
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Nucleoplasmins
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Oligoribonucleotides
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Peptides
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RNA, Small Interfering
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Tumor Necrosis Factor-alpha