Creation of a large deletion mutant of Campylobacter jejuni reveals that the lipooligosaccharide gene cluster is not required for viability

J Bacteriol. 2009 Apr;191(7):2392-9. doi: 10.1128/JB.01397-08. Epub 2009 Jan 30.

Abstract

Deletion of the lipooligosaccharide biosynthesis region (Cj1132c to Cj1152c) from the genome of Campylobacter jejuni NCTC11168 shows that the core is not required for viability. The mutant was attenuated for growth and has increased sensitivity to antibiotics and detergents. Natural transformation and invasion of cultured host cells was abolished.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biosynthetic Pathways
  • Campylobacter jejuni / chemistry
  • Campylobacter jejuni / genetics*
  • Campylobacter jejuni / growth & development
  • Campylobacter jejuni / metabolism
  • Gene Deletion*
  • Lipopolysaccharides / biosynthesis*
  • Lipopolysaccharides / chemistry
  • Microbial Viability*
  • Multigene Family*
  • Mutation

Substances

  • Bacterial Proteins
  • Lipopolysaccharides
  • lipid-linked oligosaccharides