Detection of specific bioparticles using antibodies-coated gold nanoparticles and dielectrophoretic impedance measurement

Jung-Tang Huang; Hua-Wei Yu; Shao-Yi Hou; Shiuh-Bin Fang; Hung-Chan Lee; Chu-Zen Yang

doi:10.1109/IEMBS.2008.4649878

Detection of specific bioparticles using antibodies-coated gold nanoparticles and dielectrophoretic impedance measurement

Annu Int Conf IEEE Eng Med Biol Soc. 2008:2008:3174-7. doi: 10.1109/IEMBS.2008.4649878.

Authors

Jung-Tang Huang¹, Hua-Wei Yu, Shao-Yi Hou, Shiuh-Bin Fang, Hung-Chan Lee, Chu-Zen Yang

Affiliation

¹ Department of Mechanical Engineering, National Taipei University of Technology, Taipei, Taiwan ROC. jthuang@ntut.edu.tw

PMID: 19163381
DOI: 10.1109/IEMBS.2008.4649878

Abstract

In this study, we developed an easy and quick test capable of identifying specific bacteria in one hour. The protocol was established based on the measurement of bacteria quantity on a biochip with comb-like electrodes. Gold nanoparticles coated with anti-Salmonella antibody were used to enhance the dielectrophretic property of Salmonella spp. on the biochip to facilitate the sensitivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Antibodies / analysis*
Antibodies / chemistry
Biosensing Techniques / methods*
Electric Impedance
Electrochemistry / methods
Electrodes
Gold / chemistry
Humans
Metal Nanoparticles / chemistry
Models, Statistical
Nanoparticles / chemistry*
Nanotechnology / methods*
Salmonella / metabolism
Stem Cells
Time Factors

Substances

Antibodies
Gold