Performance of microcalorimetry for early detection of methicillin resistance in clinical isolates of Staphylococcus aureus

J Clin Microbiol. 2009 Mar;47(3):774-6. doi: 10.1128/JCM.02374-08. Epub 2009 Jan 21.

Abstract

We describe a calorimetric assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA) within 5 h. Microbial heat was calculated in culture with and without cefoxitin. Among 30 genetically distinct clinical isolates, 19/20 MRSA (95%) and 10/10 methicillin-susceptible Staphylococcus aureus (100%) were correctly identified. Microcalorimetry may be useful for rapid MRSA screening.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Calorimetry / methods*
  • Cefoxitin / pharmacology
  • Humans
  • Methicillin Resistance*
  • Microbial Sensitivity Tests / methods
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / isolation & purification
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Cefoxitin