[Curcumin inhibiting the expression of indoleamine 2,3-dioxygenase induced by IFN-gamma in cancer cells]

Zhong Yao Cai. 2008 Aug;31(8):1207-11.
[Article in Chinese]

Abstract

Objective: To investigate the effect of curcumin on indoleamine 2, 3 -dioxygenase (IDO) expression induced by IFN-gamma in cancer cells.

Methods: A431, HeLa, HepG2, CNE2 cells were treated with curcumin for 24 hours, then the cell proliferation was detected by methyl thiazolyl tetrazolium (MTF) assay. The effects of curcumin on IDO expression induced by IFN-gamma in these cancer cells were demonstrated by Western blot. The transcription of interferon responsive factor-1 (IRF-1), which was a key transcription factor regulating IDO expression, was analyzed by reverse transcription polymerase chain reaction (RT-PCR) under the treatment of curcumin.

Results: Curcumin inhibited the expression of IDO in these cancer cells. However, curcumin did not inhibit the transcription of IRF-1 in cancer cells.

Conclusion: Curcumin could inhibit the expression of IDO in cancer cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cell Proliferation / drug effects
  • Curcuma / chemistry*
  • Curcumin / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation, Enzymologic / drug effects
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Indoleamine-Pyrrole 2,3,-Dioxygenase / metabolism*
  • Interferon Regulatory Factor-1 / metabolism
  • Interferon-gamma / pharmacology
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Enzyme Inhibitors
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • Interferon Regulatory Factor-1
  • Interferon-gamma
  • Curcumin