Fingerprint analysis of anti-tumor active polypeptides from Arca subcrenata by HPLC

Zhong Yao Cai. 2008 Aug;31(8):1134-8.

Abstract

RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30 degrees C; wavelength: 280 nm; flow rate: 1.0 ml/min; Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid (TFA) and solvent B contained 0.1% TFA. In this condition, ten samples' fingerprints were gained, in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetonitriles / chemistry
  • Animals
  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology
  • Bivalvia / chemistry*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Peptides / chemistry*
  • Peptides / isolation & purification
  • Peptides / pharmacology
  • Solvents / chemistry
  • Temperature

Substances

  • Acetonitriles
  • Antineoplastic Agents
  • Peptides
  • Solvents