Abstract
RP-HPLC was applied to analyze active polypeptides in Arca subcrenata, and the optimal condition for separation was also set up: temperature: 30 degrees C; wavelength: 280 nm; flow rate: 1.0 ml/min; Solvent A consisted of 80% acetonitrile and 0.1% trifluoroacetic acid (TFA) and solvent B contained 0.1% TFA. In this condition, ten samples' fingerprints were gained, in one of which the genuine fraction exhibited fourteen "common peaks" representing the characteristics of the constituents.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetonitriles / chemistry
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Animals
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology
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Bivalvia / chemistry*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Chromatography, High Pressure Liquid / methods*
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Humans
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Peptides / chemistry*
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Peptides / isolation & purification
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Peptides / pharmacology
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Solvents / chemistry
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Temperature
Substances
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Acetonitriles
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Antineoplastic Agents
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Peptides
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Solvents