Abstract
Cytoplasmic Na/K imbalance induced by cytotoxic substances (ethylene glycol and cytochalasin B) was studied. Incubation in Dulbecco's medium with these cytotoxins caused changes in K and Na concentrations in the mouse two-cell embryo blastomer. The effects of both substances resulted in a drop of Na content in the embryonic cell. Washing from cytochalasin B restored the initial Na/K balance in the cytoplasm. Possible adaptive mechanisms involved in the regulation of intracellular ionic homeostasis are discussed.
MeSH terms
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Algorithms
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Animals
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Cytochalasin B / pharmacology
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Cytotoxins / pharmacology*
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Embryo, Mammalian
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Embryonic Development / drug effects
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Embryonic Stem Cells / drug effects*
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Embryonic Stem Cells / metabolism
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Ethylene Glycol / pharmacology
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Homeostasis / drug effects*
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Intracellular Fluid / chemistry*
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Intracellular Fluid / drug effects
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Intracellular Fluid / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Potassium / analysis*
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Potassium / metabolism
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Sodium / analysis*
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Sodium / metabolism
Substances
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Cytotoxins
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Cytochalasin B
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Sodium
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Ethylene Glycol
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Potassium