Vasorelaxant and antioxidant activities are important in the therapy for cardiovascular diseases. We aimed at investigating the vasorelaxant and antioxidant activities of six xanthones isolated from Brazilian medicinal plants. Xanthone ( 1), 1-hydroxyxanthone ( 2), 4-hydroxyxanthone ( 3), 1-hydroxy-8-methoxyxanthone ( 4), 1,3-dihydroxy-7-methoxyxanthone ( 5) and 2,6,8-trihydroxy-1-methoxyxanthone ( 6) induced concentration-dependent vasorelaxant effects in endothelium-intact mice aortic rings. The presence of a hydroxy group in position 1 seemed to decrease the vasodilator effect while a hydroxy in position 4 and an increased number of hydroxy groups improved the vasorelaxatory potential of xanthones. All xanthones showed antioxidant activity but their potencies did not correlate with the vasodilator effect. Our results suggest that the tested xanthones are potentially vasorelaxant and antioxidant compounds but the two activities are not interrelated.