Agrobacterium rhizogenes pRi8196 T-DNA: mapping and DNA sequence of functions involved in mannopine synthesis and hairy root differentiation

Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7763-7. doi: 10.1073/pnas.88.17.7763.

Abstract

This paper presents the map and DNA sequence analysis of pRi8196 transferred DNA (T-DNA) genes encoding root-inducing and mannopine synthesis functions. A canonical 24-base-pair border repeat as well as two "pseudoborders" are present at the functional right T-DNA border. To the left of this border are homologs of the mas1' and mas2' genes of TR pRiA4. Next to these are five open reading frames (ORFs) homologous to ORFs 10-14 of TL of pRiA4. ORFs 10-12 (rolA, rolB, and rolC) are less related to their pRiA4 homologs than are the other large ORFs analyzed here. In contrast to T-DNA genes of pRiA4, pRi8196 T-DNA ORFs 11 and 12 (rolB and rolC) are sufficient to induce hairy roots on carrot disks.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Genes, Bacterial*
  • Mannitol / analogs & derivatives*
  • Mannitol / metabolism
  • Molecular Sequence Data
  • Open Reading Frames
  • Plants / microbiology
  • Plasmids*
  • Restriction Mapping
  • Rhizobium / genetics*
  • Rhizobium / metabolism
  • Rhizobium / physiology

Substances

  • DNA, Bacterial
  • T-DNA
  • Mannitol
  • mannopine

Associated data

  • GENBANK/M60490
  • GENBANK/M63432
  • GENBANK/M93099
  • GENBANK/M94195
  • GENBANK/M94196
  • GENBANK/M94197
  • GENBANK/M94198
  • GENBANK/M94199
  • GENBANK/M94200
  • GENBANK/X53420