Characterization of different diamond-like carbon electrodes for biosensor design

R Maalouf; H Chebib; Y Saikali; O Vittori; M Sigaud; F Garrelie; C Donnet; N Jaffrezic-Renault

doi:10.1016/j.talanta.2006.10.025

Characterization of different diamond-like carbon electrodes for biosensor design

Talanta. 2007 Apr 15;72(1):310-4. doi: 10.1016/j.talanta.2006.10.025. Epub 2006 Dec 4.

Authors

R Maalouf¹, H Chebib, Y Saikali, O Vittori, M Sigaud, F Garrelie, C Donnet, N Jaffrezic-Renault

Affiliation

¹ Center of Electrical Engineering of Lyon, CEGELY, UMR/CNRS 5005, Ecole Centrale Lyon, 69134 Ecully Cedex, France; Laboratory of Chemistry, Lebanese University, Fanar, Beirut, Lebanon; Laboratory of Analytical Electrochemistry, Claude Bernard University, Lyon I, 43 Boulevard du 11 Novembre 1918, Villeurbanne Cedex, France.

PMID: 19071620
DOI: 10.1016/j.talanta.2006.10.025

Abstract

Diamond-like carbon (DLC) films are gaining big interest in electrochemistry research area. DLC electrodes made with different ratio of sp(3)/sp(2) carbon hybridization or doped with different percentages of nickel were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for the elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutaraldehyde. Measurements were made at a fixed potential+1.0V in 40mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose, 0.6875muA/mM, than SiCNi5%, 0.3654muA/mM. Detections limits were 20muM and 30muM, respectively. Apparent Michaelis-Menten constants were found around 3mM. Forty-eight percent and 79% of the original response for 0.5mM glucose remained after 10 days for both biosensors, respectively.