[Rapid detection method of three types of bacterial pathogens in aquatic products established by multiplex PCR]

Wei Sheng Yan Jiu. 2008 Sep;37(5):602-5.
[Article in Chinese]

Abstract

Objective: To develop a rapid multiplex PCR (m-PCR) assay for simultaneously detection of three foodborne pathogens in aquatic products.

Methods: The invasion protein gene (invA) of Salmonella spp., toxR gene (toxR) of Vibrio parahaemolyticus and invasion-associated protein p60 gene (iap) of Listeria monocytogenes were used as the gene targets.

Results: The multiplex PCR assay could be specific and rapid, and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37 degrees C for 10 h.

Conclusion: The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Food Contamination / analysis
  • Food Microbiology
  • Listeria monocytogenes / isolation & purification*
  • Multiplex Polymerase Chain Reaction / methods*
  • Salmonella / isolation & purification*
  • Seafood / microbiology*
  • Sensitivity and Specificity
  • Vibrio parahaemolyticus / isolation & purification*