From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel

J Vis Exp. 2008 Jun 9:(16):831. doi: 10.3791/831.

Abstract

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Collagen*
  • Drug Combinations
  • Embryo, Mammalian
  • Embryonic Stem Cells / cytology*
  • Fibroblasts / cytology*
  • Humans
  • Laminin*
  • Mice
  • Proteoglycans*

Substances

  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen