From MEFs to Matrigel 3: passaging hESCs from Matrigel onto Matrigel

Jin Zhang; Ivan Khvorostov; Michael Teitell

doi:10.3791/832

From MEFs to Matrigel 3: passaging hESCs from Matrigel onto Matrigel

J Vis Exp. 2008 Jun 10:(16):832. doi: 10.3791/832.

Authors

Jin Zhang¹, Ivan Khvorostov, Michael Teitell

Affiliation

¹ David Geffen School of Medicine, University of California, Los Angeles, USA.

PMID: 19066542
PMCID: PMC2956217
DOI: 10.3791/832

Abstract

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.

Publication types

Research Support, Non-U.S. Gov't
Video-Audio Media

MeSH terms

Animals
Cell Culture Techniques / methods*
Collagen*
Drug Combinations
Embryo, Mammalian
Embryonic Stem Cells / cytology*
Fibroblasts / cytology*
Humans
Laminin*
Mice
Proteoglycans*

Substances

Drug Combinations
Laminin
Proteoglycans
matrigel
Collagen