Development of an immunoglobulin M capture-based enzyme-linked immunosorbent assay for diagnosis of acute infections with Bartonella henselae

Clin Vaccine Immunol. 2009 Feb;16(2):282-4. doi: 10.1128/CVI.00305-08. Epub 2008 Dec 3.

Abstract

We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.

Publication types

  • Evaluation Study

MeSH terms

  • Angiomatosis, Bacillary / diagnosis
  • Angiomatosis, Bacillary / microbiology
  • Antibodies, Bacterial / blood*
  • Bartonella henselae / immunology
  • Bartonella henselae / isolation & purification*
  • Cat-Scratch Disease / diagnosis*
  • Cat-Scratch Disease / microbiology
  • Child
  • Endocarditis, Bacterial / diagnosis
  • Endocarditis, Bacterial / microbiology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Immunoglobulin M / blood*
  • Sensitivity and Specificity
  • Young Adult

Substances

  • Antibodies, Bacterial
  • Immunoglobulin M