A system for high-speed microinjection of adherent cells

Rev Sci Instrum. 2008 Oct;79(10):104302. doi: 10.1063/1.3006000.

Abstract

This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision XY stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7% and a success rate of 82.4% (n=1012). The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / cytology
  • Cell Adhesion
  • Cell Survival
  • Cells, Cultured
  • Coloring Agents / metabolism
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Equipment Design
  • Fluorescent Dyes / metabolism
  • Imaging, Three-Dimensional
  • Kinetics
  • Microinjections / methods*
  • Micromanipulation
  • Microscopy, Confocal
  • Models, Biological
  • Quantum Dots
  • Robotics / methods*
  • Swine
  • Trypan Blue / metabolism
  • Xanthenes / metabolism

Substances

  • Coloring Agents
  • Fluorescent Dyes
  • Xanthenes
  • Texas red
  • Trypan Blue