Development and evaluation of a recombinant CP23 antigen-based ELISA for serodiagnosis of Cryptosporidium parvum

Exp Parasitol. 2009 Feb;121(2):157-62. doi: 10.1016/j.exppara.2008.10.015. Epub 2008 Nov 7.

Abstract

The CP23 gene of Cryptosporidium parvum strain isolated from Changchun in China was expressed in Escherichia coli BL21 strain and was purified as a recombinant protein. An indirect ELISA assay (CP23-ELISA) for antibody detection was established using the purified recombinant CP23 protein. Antigen coating conditions and serum dilution for the CP23-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the CP23-ELISA to evaluate the serum antibody level of the field cow samples. It indicated that the CP23-ELISA assay, which was more convenient and easier to prepare than traditional methods, was a good candidate for evaluation of C. parvum exposure to domestic animal in field.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / blood*
  • Antibodies, Helminth / immunology
  • Antigens, Protozoan* / immunology
  • Antigens, Protozoan* / isolation & purification
  • Blotting, Western / veterinary
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / immunology
  • Cattle Diseases / parasitology
  • Cryptosporidiosis / diagnosis
  • Cryptosporidiosis / parasitology
  • Cryptosporidiosis / veterinary*
  • Cryptosporidium parvum / immunology*
  • Cryptosporidium parvum / isolation & purification
  • Electrophoresis, Polyacrylamide Gel / veterinary
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / standards
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Feces / parasitology
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Sensitivity and Specificity

Substances

  • Antibodies, Helminth
  • Antigens, Protozoan
  • Recombinant Proteins