Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Han-Wen Guo; Chien-Tsun Chen; Yau-Huei Wei; Oscar K Lee; Vladimir Gukassyan; Fu-Jen Kao; Hsing-Wen Wang

doi:10.1117/1.2990752

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

J Biomed Opt. 2008 Sep-Oct;13(5):050505. doi: 10.1117/1.2990752.

Authors

Han-Wen Guo¹, Chien-Tsun Chen, Yau-Huei Wei, Oscar K Lee, Vladimir Gukassyan, Fu-Jen Kao, Hsing-Wen Wang

Affiliation

¹ Institute of Biophotonics, National Yang-Ming University, 155 Li-Nong Street, Section 2, Taipei 11221, Taiwan.

PMID: 19021377
DOI: 10.1117/1.2990752

Abstract

The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism*
Microscopy, Fluorescence / methods*
NAD / analysis*
Osteoblasts / cytology*
Osteoblasts / metabolism*
Osteogenesis / physiology*
Oxidation-Reduction
Spectrometry, Fluorescence / methods*

Substances

NAD