Simultaneous extraction and clean-up of polychlorinated biphenyls and their metabolites from small tissue samples using pressurized liquid extraction

J Chromatogr A. 2008 Dec 19;1214(1-2):37-46. doi: 10.1016/j.chroma.2008.10.089. Epub 2008 Oct 31.

Abstract

A pressurized liquid extraction-based method for the simultaneous extraction and in situ clean-up of polychlorinated biphenyls (PCBs), hydroxylated (OH)-PCBs and methylsulfonyl (MeSO(2))-PCBs from small (<0.5 g) tissue samples was developed and validated. Extraction of a laboratory reference material with hexane-dichloromethane-methanol (48:43:9, v/v) and Florisil as fat retainer allowed an efficient recovery of PCBs (78-112%; RSD: 13-37%), OH-PCBs (46+/-2%; RSD: 4%) and MeSO(2)-PCBs (89+/-21%; RSD: 24%). Comparable results were obtained with an established analysis method for PCBs, OH-PCBs and MeSO(2)-PCBs.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Aroclors / administration & dosage
  • Chemical Fractionation / methods*
  • Chlorodiphenyl (54% Chlorine) / administration & dosage
  • Linear Models
  • Liver / chemistry*
  • Male
  • Polychlorinated Biphenyls / analysis*
  • Polychlorinated Biphenyls / isolation & purification*
  • Pressure
  • Rats
  • Rats, Sprague-Dawley
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Solvents / chemistry

Substances

  • Aroclors
  • Solvents
  • Chlorodiphenyl (54% Chlorine)
  • aroclor 1242
  • Polychlorinated Biphenyls