Abstract
The first molecular epidemiology study of human immunodeficiency virus type 1 (HIV-1) in Panama has been performed with plasma samples from 66 AIDS patients infected by different transmission routes and obtained from distinct locations. All samples were amplified by RT-PCR and sequenced in gag (p17) and env (C2-C4) genes. Phylogenetic analyses revealed that 64 (97%) of the samples belong to subtype B. We also identified the presence of two CRF, one CRF12_BF and one CRF02_AG. The most notable feature of the subtype B epidemic in Panama was the large genetic distance among isolates with a mean of 15.2% but reaching up to 31.3 % in env, indicating an epidemic with a long period of evolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acquired Immunodeficiency Syndrome / epidemiology*
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Acquired Immunodeficiency Syndrome / virology*
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Adult
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Aged
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Cluster Analysis
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Female
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Genotype
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HIV-1 / classification*
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HIV-1 / genetics*
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HIV-1 / isolation & purification
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Humans
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Male
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Middle Aged
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Molecular Epidemiology
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Molecular Sequence Data
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Panama / epidemiology
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Phylogeny
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Plasma / virology
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology
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env Gene Products, Human Immunodeficiency Virus / genetics
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gag Gene Products, Human Immunodeficiency Virus / genetics
Substances
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RNA, Viral
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env Gene Products, Human Immunodeficiency Virus
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gag Gene Products, Human Immunodeficiency Virus