This study was performed to examine normal brainstem auditory evoked potential (BAEP) data for adult Japanese Black cattle and to evaluate whether differences exist in the peak latencies, interpeak latencies (IPL) and waveforms of BAEP between Japanese Black and Holstein cattle. The peaks were detected as major waves I, II, III and V in each group. The threshold of the BAEP waves in the Holstein cattle was 65-75 dB nHL, but the threshold in the Japanese Black cattle was 75-85 dB nHL. The I-III and I-V IPLs were significantly shorter in the Japanese Black compared with the Holstein cattle at an intensity of 105 dB nHL. The present findings suggest that the IPL and wave threshold of BAEP are influenced by bovine breed.