Synthesis of a heptasaccharide fragment of the mannan from Candida guilliermondii cell wall and its conjugate with BSA

Carbohydr Res. 2009 Jan 5;344(1):29-35. doi: 10.1016/j.carres.2008.09.016. Epub 2008 Sep 24.

Abstract

The 3-aminopropyl glycoside of a heptasaccharide fragment of the cell wall mannan from Candida guilliermondii 18, which corresponds to the antigenic Factor 9, has been synthesized by a convergent approach based on glycosylation of a tetrasaccharide acceptor with a trisaccharide donor as the key step to give a protected heptasaccharide 17. Subsequent two-step deprotection of 17 afforded the heptamannoside 18, which was then conjugated with BSA using the squarate procedure.

MeSH terms

  • Candida / chemistry*
  • Carbohydrate Sequence
  • Cell Wall / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mannans / chemical synthesis*
  • Mannans / chemistry
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry
  • Serum Albumin, Bovine / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Mannans
  • Oligosaccharides
  • Serum Albumin, Bovine