Nanometric layers for direct, signal-on, selective, and sensitive electrochemical detection of oligonucleotides hybridization

Grégory March; Vincent Noël; Benoît Piro; Steeve Reisberg; Minh-Chau Pham

doi:10.1021/ja8047255

Nanometric layers for direct, signal-on, selective, and sensitive electrochemical detection of oligonucleotides hybridization

J Am Chem Soc. 2008 Nov 26;130(47):15752-3. doi: 10.1021/ja8047255.

Authors

Grégory March¹, Vincent Noël, Benoît Piro, Steeve Reisberg, Minh-Chau Pham

Affiliation

¹ Interfaces, Traitement, Organisation et Dynamiques des Systèmes, Université Paris 7-Denis Diderot, UMR 7086, 15 rue Jean de Baif, 75205 Paris cedex 13, France.

PMID: 18973298
DOI: 10.1021/ja8047255

Abstract

We report a signal-on, reagentless electrochemical DNA biosensor, based on an electroactive self-assembled naphthoquinone derivative (JUG(thio)) monolayer. This system achieves highly sensitive (approximately 300 pM) and selective signal-on detection. Before hybridization, the single strand can interact with JUG(thio) and slow down the redox reaction. When the complementary target is added, the formation of the double helix eliminates the single strand/JUG(thio) interactions and the JUG(thio) redox rate, and hence the current increase.

MeSH terms

Electrochemical Techniques / methods*
Molecular Structure
Nanostructures / chemistry*
Nucleic Acid Hybridization / methods*
Oligonucleotides / analysis*
Oligonucleotides / chemistry*
Sensitivity and Specificity

Substances

Oligonucleotides