Solid phase extraction and HPLC determination of veterinary pharmaceuticals in wastewater

Sandra Babić; Danijela Asperger; Dragana Mutavdzić; Alka J M Horvat; Marija Kastelan-Macan

doi:10.1016/j.talanta.2006.07.003

Solid phase extraction and HPLC determination of veterinary pharmaceuticals in wastewater

Talanta. 2006 Nov 15;70(4):732-8. doi: 10.1016/j.talanta.2006.07.003. Epub 2006 Aug 22.

Authors

Sandra Babić¹, Danijela Asperger, Dragana Mutavdzić, Alka J M Horvat, Marija Kastelan-Macan

Affiliation

¹ Laboratory of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 20, HR-10000 Zagreb, Croatia.

PMID: 18970832
DOI: 10.1016/j.talanta.2006.07.003

Abstract

This work focuses on the application of SPE-HPLC analysis of important veterinary pharmaceuticals from different classes in highly complex wastewater matrix. The pharmaceutical investigated included three sulfonamides (sulfamethazine, sulfadiazine and sulfaguanidine), a sulfonamide synergist (trimethoprim), a tetracycline (oxytetracycline), a fluoroquinolone (enrofloxacine) and a beta-lactame (penicillin G/procaine). The method involves pre-concentration and clean-up by solid phase extraction (SPE) using Oasis HLB extraction catridges. Final analysis of the selected pharmaceutical compounds was carried out by high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD). Recoveries were ranged from 68.3 to 97.9% with relative standard deviation below 8.4%. Only for sulfaguanidine low recovery was obtained. Limits of quantification were in the range 1.5-100mug/L depending on pharmaceutical. The described method was applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.