A new method for determination of uric acid by the lactic acid-acetone-BrO(3)(-)-Mn(2+)-H(2)SO(4) oscillating reaction using the analyte pulse perturbation technique

Jun Wang; Shutao Yang; Ruxiu Cai; Zhixin Lin; Zhihong Liu

doi:10.1016/j.talanta.2004.08.008

A new method for determination of uric acid by the lactic acid-acetone-BrO(3)(-)-Mn(2+)-H(2)SO(4) oscillating reaction using the analyte pulse perturbation technique

Talanta. 2005 Feb 15;65(3):799-805. doi: 10.1016/j.talanta.2004.08.008.

Authors

Jun Wang¹, Shutao Yang, Ruxiu Cai, Zhixin Lin, Zhihong Liu

Affiliation

¹ College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.

PMID: 18969871
DOI: 10.1016/j.talanta.2004.08.008

Abstract

A new analytical method for the determination of uric acid (UA) by the perturbation of UA on the Belousov-Zhabotinsky oscillating reaction is proposed. The method is based on the linear relationship between the changes in the oscillating period and the concentration of UA. The calibration curve is linear over the range of 2.0 x 10(-5) to 5.0 x 10(-4)M, with a detecting limit of 3.28 x 10(-6)M. The method features good precision (R.S.D.: 3.59%) and excellent throughput (10samplesh(-1)). The possible mechanism of the perturbation of UA on the oscillating reaction is discussed.