Voltammetric determination of phytochelatins using copper solid amalgam electrode

B Yosypchuk; I Sestáková; L Novotný

doi:10.1016/S0039-9140(03)00023-7

Voltammetric determination of phytochelatins using copper solid amalgam electrode

Talanta. 2003 May 1;59(6):1253-8. doi: 10.1016/S0039-9140(03)00023-7.

Authors

B Yosypchuk¹, I Sestáková, L Novotný

Affiliation

¹ J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejskova 3, 18223 Prague 8, Czech Republic.

PMID: 18969016
DOI: 10.1016/S0039-9140(03)00023-7

Abstract

Voltammetric determination of synthetically prepared phytochelatins (gamma-Glu-Cys)(2)Gly (PC(2)) and (gamma-Glu-Cys)(3)Gly (PC(3)) has been studied using new type of copper solid amalgam electrode. The determination, based on the formation of cuprous complexes in buffer pH 8.1, is suitable for concentrations of PC in the range 10-100 nmol l(-1). Reproducibility, employing electrochemical cleaning of the electrode surface, was statistically evaluated. The achieved limit of detection (2.1-2.6x10(-9) mol l(-1) for DCV measurement) together with the robust character of the electrode offer its use for detection of PCs in separated extracts of real samples.