Evaluation of antioxidative capability of the tomato (Solanum lycopersicum) skin constituents by capillary electrophoresis and high-performance liquid chromatography

Electrophoresis. 2008 Oct;29(19):3980-8. doi: 10.1002/elps.200800012.

Abstract

In the current study, phenolic compounds of the tomato (Solanum lycopersicum) skin extract were separated and their composition was determined by capillary electrophoresis and tandem high-performance liquid chromatography diode array detection-mass spectrometry (HPLC-DAD-MS/MS). Both the total phenolic and flavonoid contents of the extract were determined. The antioxidative capability of the extract was measured using a stable free radical 2,2-diphenyl-1-picrylhydrazyl assay. The monitoring of the radical scavenging capability of specific phenolic compounds was carried out both by capillary electrophoresis and HPLC-MS/MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis
  • Antioxidants / metabolism*
  • Biphenyl Compounds / analysis
  • Biphenyl Compounds / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Electrophoresis, Capillary*
  • Flavonoids / analysis
  • Flavonoids / metabolism
  • Phenols / analysis
  • Phenols / metabolism
  • Picrates / analysis
  • Picrates / metabolism
  • Plant Epidermis / chemistry
  • Plant Extracts / analysis
  • Plant Extracts / metabolism*
  • Solanum lycopersicum / chemistry*
  • Spectrophotometry, Ultraviolet
  • Tandem Mass Spectrometry

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Flavonoids
  • Phenols
  • Picrates
  • Plant Extracts
  • 1,1-diphenyl-2-picrylhydrazyl