Abstract
PhiRSB1 is a wide-host-range, T7-like bacteriophage that infects and efficiently lyses the phytopathogenic bacterium Ralstonia solanacearum. The phiRSB1 genome comprises 43,079 bp of double-stranded DNA (61.7% G+C) with 325-bp terminal repeats and contains 47 open reading frames. Strong activity of tandem early promoters and wide specificity of phage promoters of phiRSB1 were demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriophage T7 / classification
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Bacteriophage T7 / genetics
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Bacteriophages / classification
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Bacteriophages / genetics*
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Bacteriophages / ultrastructure
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DNA, Viral / genetics
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Genome, Viral
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Molecular Sequence Data
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Open Reading Frames
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Podoviridae / classification
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Podoviridae / genetics
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Promoter Regions, Genetic
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Ralstonia solanacearum / virology*
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Transcription, Genetic
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Viral Plaque Assay
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Viral Proteins / genetics
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Virion / genetics
Substances
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DNA, Viral
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Viral Proteins