Abstract
The molecular properties of immunosuppressive n-3 polyunsaturated fatty acids (PUFA) have not been fully elucidated. Using CD4(+) T cells from wild-type control and fat-1 transgenic mice (enriched in n-3 PUFA), we show that membrane raft accumulation assessed by Laurdan (6-dodecanoyl-2-dimethyl aminonaphthalene) labeling was enhanced in fat-1 cells following immunological synapse (IS) formation by CD3-specific Ab expressing hybridoma cells. However, the localization of protein kinase Ctheta, phospholipase Cgamma-1, and F-actin into the IS was suppressed. In addition, both the phosphorylation status of phospholipase Cgamma-1 at the IS and cell proliferation as assessed by CFSE labeling and [(3)H]thymidine incorporation were suppressed in fat-1 cells. These data imply that lipid rafts may be targets for the development of dietary agents for the treatment of autoimmune and chronic inflammatory diseases.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / chemistry
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / metabolism*
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Caenorhabditis elegans Proteins / biosynthesis
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism
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Cell Proliferation
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Cells, Cultured
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Coculture Techniques
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Fatty Acid Desaturases / biosynthesis
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Fatty Acid Desaturases / genetics
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Fatty Acid Desaturases / metabolism
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Fatty Acids, Omega-3 / physiology*
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Immunological Synapses / chemistry
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Immunological Synapses / metabolism*
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Intracellular Signaling Peptides and Proteins / antagonists & inhibitors*
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Intracellular Signaling Peptides and Proteins / metabolism*
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Intracellular Signaling Peptides and Proteins / physiology
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Lymphocyte Activation / immunology
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Membrane Microdomains / chemistry
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Membrane Microdomains / immunology*
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Membrane Microdomains / metabolism*
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Models, Biological
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Phosphorylation / immunology
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Protein Transport / immunology
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Signal Transduction / immunology*
Substances
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Caenorhabditis elegans Proteins
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Fatty Acids, Omega-3
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Intracellular Signaling Peptides and Proteins
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fat-1 protein, C elegans
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Fatty Acid Desaturases